The Human SEPP1 (Selenoprotein P) Plasma CLIA Kit is a highly reliable and accurate tool for quantitative detection of SEPP1 levels in human plasma samples. This kit is specifically designed to offer high sensitivity and specificity, ensuring consistent and reproducible results for your research needs.SEPP1, also known as Selenoprotein P, is an important protein involved in selenium transport and antioxidant defense mechanisms in the body. Abnormal SEPP1 levels have been associated with various health conditions, including thyroid disorders, cardiovascular diseases, and cancer.
Therefore, precise measurement of SEPP1 levels can provide valuable insights into these conditions and potentially guide the development of therapeutic interventions.With the Human SEPP1 Plasma CLIA Kit, researchers can easily and accurately quantify SEPP1 levels in human plasma samples, allowing for in-depth investigation of its role in health and disease. Trust in this kit to deliver reliable results for your research endeavors.
Product Name:
Human SEPP1 (Selenoprotein P, Plasma) CLIA Kit
SKU:
HUES01150
Target:
Human SEPP1 (Selenoprotein P, Plasma)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SEPP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SEPP1. You can calculate the concentration of Human SEPP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
103-119
84-96
Average (%)
93
109
90
1:4
Range (%)
90-105
91-105
90-102
Average (%)
96
97
95
1:8
Range (%)
99-116
87-101
85-100
Average (%)
106
94
91
1:16
Range (%)
97-109
101-118
98-116
Average (%)
103
109
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
99
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.82
307.53
783.39
98.28
330.51
761.68
Standard deviation
12.04
22.7
75.28
10.09
25.58
56.14
C V (%)
11.17
7.38
9.61
10.27
7.74
7.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SEPP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SEPP1 in samples. No significant cross-reactivity or interference between Human SEPP1 and analogues was observed.
