Human SFRP2 (Secreted Frizzled Related Protein 2) CLIA Kit
The Human SFRP2 (Secreted Frizzled-Related Protein 2) CLIA Kit is specially designed for the precise measurement of SFRP2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.SFRP2 is a key protein involved in various biological processes, including cell migration, tissue development, and wound healing. Dysregulation of SFRP2 has been linked to conditions such as cancer, cardiovascular diseases, and fibrosis, highlighting its importance as a potential biomarker for disease detection and therapeutic development.
By using the Human SFRP2 CLIA Kit, researchers can effectively study the role of SFRP2 in different pathologies and explore its potential as a target for novel treatments. This kit provides a valuable tool for advancing scientific understanding and advancing medical progress in various fields of research.
Product Name:
Human SFRP2 (Secreted Frizzled Related Protein 2) CLIA Kit
SKU:
HUES00758
Target:
Human SFRP2 (Secreted Frizzled Related Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SFRP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SFRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SFRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SFRP2. You can calculate the concentration of Human SFRP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
90-103
95-109
Average (%)
97
97
102
1:4
Range (%)
97-110
86-101
96-109
Average (%)
104
92
101
1:8
Range (%)
89-101
101-112
88-103
Average (%)
95
106
95
1:16
Range (%)
94-105
101-116
92-105
Average (%)
99
108
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-98
90
EDTA plasma (n=5)
100-117
108
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
1.92
6.77
0.99
1.81
6.55
Standard deviation
0.11
0.17
0.73
0.09
0.13
0.63
C V (%)
11.0
8.85
10.78
9.09
7.18
9.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SFRP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.
