The Human sGP130 (Soluble Glucoprotein 130) CLIA Kit is specifically designed for the accurate detection of sGP130 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.sGP130 is a crucial biomarker involved in the regulation of inflammation and immune responses. It plays a significant role in various diseases including autoimmune disorders, inflammatory conditions, and cancer.
Studying sGP130 levels can provide valuable insights into disease progression and potential treatment strategies.With its user-friendly protocol and optimized assay conditions, the Human sGP130 CLIA Kit is a valuable tool for researchers and clinicians seeking to advance their understanding of sGP130 biology and its implications in human health and disease. Explore the potential of sGP130 as a biomarker and accelerate your research with this high-quality CLIA kit from Assay Genie.
Product Name:
Human sgp130 (soluble Glucoprotein 130) CLIA Kit
SKU:
HUES00080
Target:
Human sgp130 (soluble Glucoprotein 130)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sgp130. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sgp130 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sgp130, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human sgp130. You can calculate the concentration of Human sgp130 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
92-103
95-109
Average (%)
97
98
101
1:4
Range (%)
98-113
85-96
97-110
Average (%)
105
91
102
1:8
Range (%)
88-103
96-112
95-110
Average (%)
95
102
102
1:16
Range (%)
96-113
100-114
98-116
Average (%)
103
107
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
217.84
539.72
1359.07
204.32
556.26
1245.31
Standard deviation
19.19
63.2
114.98
16.47
47.34
98.01
C V (%)
8.81
11.71
8.46
8.06
8.51
7.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human sgp130 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sgp130 in samples. No significant cross-reactivity or interference between Human sgp130 and analogues was observed.
