Human SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) ELISA Kit
The Human SH3 Domain Binding Glutamic Acid-Rich Protein Like Protein (SH3BGRL) ELISA Kit is a powerful tool for researchers studying the role of SH3BGRL in various biological processes. This kit allows for the precise measurement of SH3BGRL levels in human serum, plasma, and cell culture supernatants, providing accurate and reliable results.SH3BGRL is known to play a critical role in protein-protein interactions and intracellular signaling pathways, making it a valuable target for research in areas such as cancer, cell migration, and immune response modulation.
By accurately quantifying SH3BGRL levels, researchers can gain valuable insights into its functions and potential therapeutic applications.With its high sensitivity and specificity, the SH3BGRL ELISA Kit offers a robust and efficient solution for studying SH3BGRL biology and exploring its implications in various diseases and biological processes. Trust in this kit to deliver accurate and reproducible results, advancing your research efforts and contributing to a deeper understanding of SH3BGRL's role in human health and disease.
Product Name:
Human SH3 Domain Binding Glutamic Acid Rich Protein Like Protein (SH3BGRL) ELISA Kit
Product Code:
HUDL02669
Size:
96 Assays
Target:
SH3BGRL
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
