Human SH3 Domain Binding Protein 2 (SH3BP2) ELISA Kit
The Human SH3 Domain Binding Protein 2 (SH3BP2) ELISA Kit is an essential tool for the accurate and sensitive detection of SH3BP2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for various research applications.SH3BP2 is a key player in cell signaling and regulation, playing a crucial role in diverse cellular processes such as cell adhesion, migration, and proliferation. Dysregulation of SH3BP2 has been implicated in various diseases, including cancer, autoimmune disorders, and skeletal abnormalities.
Therefore, monitoring SH3BP2 levels using this ELISA kit can help researchers better understand the molecular mechanisms underlying these conditions and potentially identify novel therapeutic targets.Whether studying the role of SH3BP2 in disease pathogenesis or investigating its potential as a diagnostic or prognostic biomarker, the Human SH3BP2 ELISA Kit offers a reliable and efficient solution for accurate quantification of SH3BP2 levels in human samples.
Product Name:
Human SH3 Domain Binding Protein 2 (SH3BP2) ELISA Kit
Product Code:
HUDL02670
Size:
96 Assays
Target:
SH3BP2
Synonyms:
CRBM, RES4-23, Cherubism
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
