Human SHBG (Sex Hormone Binding Globulin) CLIA Kit
The Human SHBG (Sex Hormone Binding Globulin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of SHBG levels in human serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it ideal for various research applications in the field of endocrinology and hormone regulation.SHBG is a glycoprotein that binds to sex hormones such as testosterone and estradiol, regulating their availability and biological activity in the body.
Imbalances in SHBG levels have been associated with conditions such as polycystic ovary syndrome, hypothyroidism, and hormonal imbalances, making it a crucial biomarker for understanding and diagnosing these conditions.By utilizing the Human SHBG CLIA Kit, researchers can gain valuable insights into hormone regulation and its impact on various physiological processes, paving the way for advancements in the diagnosis and treatment of hormone-related disorders.
Product Name:
Human SHBG (Sex Hormone Binding Globulin) CLIA Kit
SKU:
HUES01134
Target:
Human SHBG (Sex Hormone Binding Globulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.75 nmol/L
Detection range:
1.25-80 nmol/L
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SHBG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SHBG. You can calculate the concentration of Human SHBG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
94-108
100-110
Average (%)
106
102
105
1:4
Range (%)
98-114
90-104
100-115
Average (%)
106
95
107
1:8
Range (%)
93-111
90-103
94-110
Average (%)
101
98
101
1:16
Range (%)
100-116
100-116
84-97
Average (%)
107
106
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.2
11.68
28.66
3.97
12.5
29.93
Standard deviation
0.53
0.92
3.1
0.36
1.05
2.94
C V (%)
12.62
7.88
10.82
9.07
8.4
9.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SHBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed.
