Description
Human SHBG (Sex Hormone Binding Globulin) ELISA Kit
The Human SHBG (Sex Hormone Binding Globulin) ELISA Kit is a specialized assay designed for the quantitative analysis of Sex Hormone Binding Globulin in human biological samples. SHBG, a glycoprotein produced by the liver, plays a crucial role in regulating the bioavailability and transport of sex hormones, namely testosterone and estradiol. As a carrier protein, SHBG modulates the distribution and biological activity of these hormones, impacting various physiological processes related to reproduction, metabolism, and more.
Our SHBG ELISA Kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for your research or clinical investigations. Manufactured under rigorous quality control standards, this kit delivers consistent performance and straightforward usability, making it an ideal choice for studying SHBG levels in diverse sample types. Rely on Assay Genie's SHBG ELISA Kit for the accurate and reliable quantification of this essential biomarker in your studies.
| Product Name: | Human SHBG (Sex Hormone Binding Globulin) ELISA Kit |
| Product Code: | AEES00134 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 1.56-100 pmol/mL |
| Sensitivity: | 0.94 pmol/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human SHBG concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SHBG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SHBG. You can calculate the concentration of Human SHBG in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
