The Human SHC Transforming Protein 1 (SHC1) ELISA Kit is specially designed for the precise measurement of SHC1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.SHC1 is a key protein that plays a critical role in cellular signaling pathways, including those involved in cell growth, differentiation, and survival. Dysregulation of SHC1 has been implicated in various diseases such as cancer, diabetes, and neurological disorders, highlighting its importance as a potential biomarker for understanding disease mechanisms and developing targeted therapies.
With the Human SHC1 ELISA Kit, researchers can confidently analyze SHC1 levels in human samples, providing valuable insights into the role of this protein in health and disease. These reliable and reproducible results make this kit an essential tool for advancing scientific knowledge and medical research.
Product Name:
Human SHC-Transforming Protein 1 (SHC1) ELISA Kit
Product Code:
HUDL02673
Size:
96 Assays
Target:
SHC1
Synonyms:
SHC, SHCA, p52SHC, p66, p66SHC, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
