Human SIGLEC1 (Sialic Acid Binding Ig Like Lectin 1) CLIA Kit
The Human SIGLEC1 (Sialic Acid Binding Ig-like Lectin 1) CLIA Kit is specifically designed for the accurate measurement of SIGLEC1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SIGLEC1 is a critical protein involved in immune response and cell signaling, playing a key role in the regulation of inflammatory processes and immune cell activation.
Abnormal SIGLEC1 levels have been associated with various diseases, including autoimmune disorders and infectious diseases, making it a valuable biomarker for both research and clinical diagnostics.Overall, the Human SIGLEC1 CLIA Kit provides researchers with a reliable tool for studying the role of SIGLEC1 in health and disease, offering insights into potential therapeutic targets and diagnostic markers.
Product Name:
Human SIGLEC1 (Sialic Acid Binding Ig Like Lectin 1) CLIA Kit
SKU:
HUES00762
Target:
Human SIGLEC1 (Sialic Acid Binding Ig Like Lectin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIGLEC1. You can calculate the concentration of Human SIGLEC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
99-112
102-117
Average (%)
91
105
109
1:4
Range (%)
87-101
98-112
89-100
Average (%)
94
105
94
1:8
Range (%)
100-113
88-101
95-112
Average (%)
107
95
102
1:16
Range (%)
93-110
86-102
102-117
Average (%)
101
93
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.07
204.76
699.54
110.1
217.8
637.6
Standard deviation
12.93
22.22
45.89
9.09
16.94
62.68
C V (%)
12.08
10.85
6.56
8.26
7.78
9.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SIGLEC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SIGLEC1 in samples. No significant cross-reactivity or interference between Human SIGLEC1 and analogues was observed.
