Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) CLIA Kit
The Human SIGLEC3 (Sialic acid-binding Ig-like lectin 3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of SIGLEC3 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.SIGLEC3 is a critical protein involved in immune responses and cell signaling pathways.
It plays a key role in immune cell function and regulation, making it a valuable biomarker for studying immune disorders, inflammation, and autoimmune conditions. The SIGLEC3 CLIA Kit offers researchers a powerful tool for studying the role of SIGLEC3 in health and disease, and for developing potential therapeutic interventions.
Product Name:
Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) CLIA Kit
SKU:
HUES01091
Target:
Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIGLEC3. You can calculate the concentration of Human SIGLEC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
88-99
85-100
Average (%)
95
94
91
1:4
Range (%)
88-105
98-111
84-96
Average (%)
96
105
91
1:8
Range (%)
96-112
92-108
90-105
Average (%)
103
99
96
1:16
Range (%)
98-115
97-112
89-104
Average (%)
105
104
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
102
EDTA plasma (n=5)
94-109
102
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.44
173.94
780.01
101.38
181.62
729.13
Standard deviation
11.96
19.6
75.66
8.65
13.8
81.59
C V (%)
12.03
11.27
9.7
8.53
7.6
11.19
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SIGLEC3 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human SIGLEC3 in samples. No significant cross-reactivity or interference between Human SIGLEC3 and analogues was observed.
