Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit
The Human SLAMF7 (Signaling Lymphocytic Activation Molecule Family Member 7) ELISA Kit is a powerful tool for the precise measurement of SLAMF7 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and dependable results, making it a valuable asset for a variety of research endeavors.SLAMF7 is an important member of the signaling lymphocytic activation molecule family, known for its role in immune response regulation and cell activation.
Its involvement in conditions such as autoimmune diseases, infectious diseases, and cancer underscores its significance as a potential therapeutic target and biomarker for disease progression.By utilizing the Human SLAMF7 ELISA Kit, researchers can gain valuable insights into the expression and function of SLAMF7 in various biological contexts, paving the way for new discoveries and advancements in the field of immunology and disease pathogenesis.
Product Name:
Human Signaling Lymphocytic Activation Molecule Family, Member 7 (SLAMF7) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
