Human Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit
The Human Single-Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit is a highly sensitive and specific assay used for the accurate detection of SMUG1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed to provide reliable and reproducible results, making it ideal for a variety of research applications.SMUG1 is an important enzyme that plays a critical role in DNA repair by specifically recognizing and removing uracil bases from single-stranded DNA.
Dysregulation of SMUG1 has been implicated in various diseases such as cancer, neurodegenerative disorders, and autoimmune diseases, highlighting its significance as a potential biomarker for understanding the pathogenesis of these conditions and developing targeted therapies.Overall, the Human SMUG1 ELISA Kit offers researchers a valuable tool for studying the role of SMUG1 in DNA repair mechanisms and its implications in disease pathology.
Product Name:
Human Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) ELISA Kit
Product Code:
HUDL02725
Size:
96 Assays
Target:
SMUG1
Synonyms:
FDG, HMUDG, UNG3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
