SynonymsSIR2L7,NAD-Dependent Deacetylase Sirtuin-7,Silent Mating Type Information Regulation 2 Homolog 7,Regulatory protein SIR2 homolog 7,SIR2-like protein 7
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL02693
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9NRC8
Sensitivity:
0.071ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SIR2L7,NAD-Dependent Deacetylase Sirtuin-7,Silent Mating Type Information Regulation 2 Homolog 7,Regulatory protein SIR2 homolog 7,SIR2-like protein 7
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Human SIRT7 (Sirtuin 7) ELISA Kit is specifically designed for the precise quantification of SIRT7 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring trustworthy and consistent results for various research purposes.SIRT7 is a vital member of the sirtuin protein family, playing a crucial role in regulating cellular processes such as gene expression, DNA repair, and metabolism. Dysregulation of SIRT7 has been implicated in various diseases, including cancer, metabolic disorders, and aging-related conditions, highlighting its significance as a potential therapeutic target and diagnostic marker.
By using the Human SIRT7 ELISA Kit, researchers can advance their studies on the role of SIRT7 in health and disease, paving the way for innovative treatments and interventions. Trust in this kit to deliver accurate and meaningful data to support your research endeavors.
Product Name:
Human Sirtuin 7 (SIRT7) ELISA Kit
Product Code:
HUDL02693
Size:
96 Assays
Target:
SIRT7
Synonyms:
SIR2L7, NAD-Dependent Deacetylase Sirtuin-7, Silent Mating Type Information Regulation 2 Homolog 7, Regulatory protein SIR2 homolog 7, SIR2-like protein 7
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
