Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit
The Human SMAD1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit is specifically designed for the quantitative measurement of SMAD1/MADH1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.SMAD1/MADH1 is a key transcription factor involved in the TGF-β signaling pathway, regulating important cellular processes such as growth and differentiation.
Dysregulation of SMAD1/MADH1 has been linked to various diseases, including cancer, fibrosis, and developmental disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human SMAD1/MADH1 CLIA Kit provides researchers with a powerful tool to investigate the role of SMAD1/MADH1 in disease pathogenesis and to further our understanding of molecular mechanisms underlying various disorders.
Product Name:
Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit
SKU:
HUES00555
Target:
Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad1/MADH1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad1/MADH1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad1/MADH1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Smad1/MADH1. You can calculate the concentration of Human Smad1/MADH1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
86-98
93-108
Average (%)
92
92
99
1:4
Range (%)
95-109
100-118
89-103
Average (%)
103
108
94
1:8
Range (%)
87-98
94-110
96-110
Average (%)
92
101
102
1:16
Range (%)
92-108
91-104
91-108
Average (%)
99
97
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
100-112
106
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.02
4.12
0.49
0.97
4.49
Standard deviation
0.06
0.11
0.3
0.06
0.1
0.29
C V (%)
11.76
10.78
7.28
12.24
10.31
6.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Smad1/MADH1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Smad1/MADH1 in samples. No significant cross-reactivity or interference between Human Smad1/MADH1 and analogues was observed.
