Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) CLIA Kit
The Human SMAD3 (Mothers Against Decapentaplegic Homolog 3) CLIA Kit is designed for the precise measurement of SMAD3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.SMAD3 is a key transcription factor involved in the TGF-beta signaling pathway, regulating cell growth, differentiation, and immune responses.
Dysregulation of SMAD3 has been linked to various diseases, including cancer, fibrosis, and inflammatory disorders, making it a valuable biomarker for understanding these conditions and developing potential treatments.Overall, the Human SMAD3 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying the role of SMAD3 in human health and disease, offering insights that could lead to new therapeutic approaches.
Product Name:
Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) CLIA Kit
SKU:
HUES00767
Target:
Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad3/MADH3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad3/MADH3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad3/MADH3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Smad3/MADH3. You can calculate the concentration of Human Smad3/MADH3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
101-117
89-104
Average (%)
93
109
96
1:4
Range (%)
98-112
97-111
93-105
Average (%)
105
103
99
1:8
Range (%)
93-108
93-108
93-103
Average (%)
100
100
98
1:16
Range (%)
94-106
85-96
84-97
Average (%)
100
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.01
3.87
0.56
1.06
4.07
Standard deviation
0.06
0.11
0.38
0.06
0.1
0.42
C V (%)
11.11
10.89
9.82
10.71
9.43
10.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Smad3/MADH3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Smad3/MADH3 in samples. No significant cross-reactivity or interference between Human Smad3/MADH3 and analogues was observed.
