Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4) CLIA Kit
The Human SMAD4 (Mothers Against Decapentaplegic Homolog 4) CLIA Kit is specifically designed for the precise and accurate measurement of SMAD4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.SMAD4, also known as MADH4, is a key protein involved in the TGF-β signaling pathway, playing a crucial role in cell growth, differentiation, and death.
Dysregulation of SMAD4 has been linked to various diseases, including cancer, gastrointestinal disorders, and developmental abnormalities. Therefore, the Human SMAD4 CLIA Kit serves as a valuable tool for studying the role of SMAD4 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4) CLIA Kit
SKU:
HUES00556
Target:
Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad4/MADH4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad4/MADH4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad4/MADH4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Smad4/MADH4. You can calculate the concentration of Human Smad4/MADH4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
87-103
101-114
Average (%)
102
94
107
1:4
Range (%)
91-105
95-112
89-104
Average (%)
99
103
95
1:8
Range (%)
94-111
98-109
89-101
Average (%)
101
103
94
1:16
Range (%)
84-97
99-116
84-96
Average (%)
91
107
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
104
EDTA plasma (n=5)
96-108
103
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.32
465.34
1687.45
200.65
420.11
1531.59
Standard deviation
23.24
52.58
189.84
17.24
38.4
103.23
C V (%)
12.34
11.3
11.25
8.59
9.14
6.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Smad4/MADH4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Smad4/MADH4 in samples. No significant cross-reactivity or interference between Human Smad4/MADH4 and analogues was observed.
