Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7) CLIA Kit
The Human SMAD7 (MADH7 - Mothers Against Decapentaplegic Homolog 7) CLIA Kit is specifically designed for the precise measurement of SMAD7 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for a variety of research applications.SMAD7 is a key regulator in the TGF-β signaling pathway, playing a critical role in cell growth, differentiation, and apoptosis.
Dysregulation of SMAD7 has been implicated in various diseases such as cancer, fibrosis, and inflammatory disorders, highlighting its significance as a potential biomarker for disease progression and therapeutic targeting.Overall, the Human SMAD7 CLIA Kit provides researchers with a reliable and efficient method for measuring SMAD7 levels, allowing for a better understanding of its role in disease pathogenesis and the development of novel treatment strategies.
Product Name:
Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7) CLIA Kit
SKU:
HUES00557
Target:
Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad7/MADH7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad7/MADH7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad7/MADH7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Smad7/MADH7. You can calculate the concentration of Human Smad7/MADH7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-117
84-97
92-105
Average (%)
107
90
100
1:4
Range (%)
86-100
96-109
84-98
Average (%)
92
102
91
1:8
Range (%)
86-97
98-110
86-96
Average (%)
91
103
91
1:16
Range (%)
97-112
98-112
84-99
Average (%)
104
105
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.78
217.6
784.93
100.55
203.41
846.53
Standard deviation
10.23
24.89
83.12
12.39
22.42
78.05
C V (%)
10.25
11.44
10.59
12.32
11.02
9.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Smad7/MADH7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Smad7/MADH7 in samples. No significant cross-reactivity or interference between Human Smad7/MADH7 and analogues was observed.
