Human SMN1 (Survival of Motor Neuron 1, Telomeric) CLIA Kit
The Human SMN1 (Survival of Motor Neuron 1) Telomeric CLIA Kit is a highly specialized assay designed for the precise measurement of SMN1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.SMN1 is a critical gene responsible for the survival of motor neurons, playing a crucial role in conditions like spinal muscular atrophy (SMA).
By accurately quantifying SMN1 levels, researchers can gain valuable insights into the mechanisms underlying SMA and other neurodegenerative disorders, leading to potentially groundbreaking advancements in diagnosis and treatment strategies.Equip your laboratory with the cutting-edge Human SMN1 Telomeric CLIA Kit from Assay Genie and unlock new possibilities in the study of motor neuron diseases.
Product Name:
Human SMN1 (Survival of Motor Neuron 1, Telomeric) CLIA Kit
SKU:
HUES00979
Target:
Human SMN1 (Survival of Motor Neuron 1, Telomeric)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SMN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SMN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SMN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SMN1. You can calculate the concentration of Human SMN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
95-110
100-115
Average (%)
95
101
106
1:4
Range (%)
100-118
93-104
102-118
Average (%)
108
98
109
1:8
Range (%)
93-106
83-96
100-115
Average (%)
100
90
109
1:16
Range (%)
86-100
97-112
98-115
Average (%)
93
103
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
96-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.6
143.02
358.59
52.8
139.83
323.13
Standard deviation
5.42
10.3
29.26
6.25
16.74
32.73
C V (%)
10.71
7.2
8.16
11.84
11.97
10.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SMN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SMN1 in samples. No significant cross-reactivity or interference between Human SMN1 and analogues was observed.
