The Human α-Synuclein CLIA Kit is specifically designed for the sensitive and accurate detection of α-Synuclein levels in human samples such as serum, plasma, and cell culture supernatants. This innovative kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.α-Synuclein is a key protein associated with neurodegenerative diseases such as Parkinson's and Alzheimer's, making it a vital biomarker for studying these conditions and investigating potential therapeutic interventions.
This CLIA kit provides researchers with a powerful tool for exploring the role of α-Synuclein in disease progression and developing novel treatment strategies.Overall, the Human α-Synuclein CLIA Kit is an indispensable resource for researchers seeking to delve into the complex mechanisms underlying neurodegenerative disorders, paving the way for advancements in diagnosis and treatment of these debilitating conditions.
Product Name:
Human SNC alpha (Synuclein Alpha) CLIA Kit
SKU:
HUES00581
Target:
Human SNC alpha (Synuclein Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SNCα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SNCα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SNCα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SNCα. You can calculate the concentration of Human SNCα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
92-105
100-117
Average (%)
101
100
107
1:4
Range (%)
89-104
101-118
88-103
Average (%)
96
108
95
1:8
Range (%)
92-103
96-110
90-104
Average (%)
97
103
95
1:16
Range (%)
94-107
100-118
84-97
Average (%)
102
108
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.55
71.16
322.94
39.24
70.03
319.91
Standard deviation
3.96
7.18
31.29
4.67
6.14
34.13
C V (%)
10.83
10.09
9.69
11.9
8.77
10.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SNCα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SNCα in samples. No significant cross-reactivity or interference between Human SNCα and analogues was observed.
