Human SOCS3 (Suppressors of Cytokine Signaling 3) CLIA Kit
The Human SOCS3 (Suppressors of Cytokine Signaling 3) CLIA Kit is designed for the accurate detection of suppressors of cytokine signaling 3 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying immune responses and inflammation.SOCS3 is a key regulator of cytokine signaling pathways, controlling the immune response and inflammation in various diseases including autoimmune disorders, cancer, and infectious diseases.
By measuring SOCS3 levels, researchers can gain valuable insights into the activity of these pathways and identify potential therapeutic targets.Overall, the Human SOCS3 CLIA Kit provides a valuable tool for researchers looking to study immune responses, inflammation, and related diseases, offering precise and accurate results for their experiments.
Product Name:
Human SOCS3 (Suppressors of Cytokine Signaling 3) CLIA Kit
SKU:
HUES01114
Target:
Human SOCS3 (Suppressors of Cytokine Signaling 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SOCS3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOCS3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOCS3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SOCS3. You can calculate the concentration of Human SOCS3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-97
88-98
89-101
Average (%)
90
93
95
1:4
Range (%)
102-117
101-114
90-105
Average (%)
110
107
97
1:8
Range (%)
91-106
100-113
90-101
Average (%)
98
106
95
1:16
Range (%)
91-106
101-120
96-112
Average (%)
96
110
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-115
108
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
96-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.73
79.1
215.07
25.21
85.55
234.72
Standard deviation
2.95
6.14
18.24
2.99
6.6
15.14
C V (%)
11.93
7.76
8.48
11.86
7.71
6.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SOCS3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SOCS3 in samples. No significant cross-reactivity or interference between Human SOCS3 and analogues was observed.
