Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
The Human SOD1 (Superoxide Dismutase 1) Soluble ELISA Kit is specifically designed for the accurate measurement of soluble SOD1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SOD1 is a critical antioxidant enzyme that plays a key role in neutralizing harmful free radicals in the body, protecting cells from oxidative stress and damage.
Dysregulation of SOD1 has been linked to various diseases including amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders. Therefore, the measurement of SOD1 levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Human SOD1 Soluble ELISA Kit is a valuable tool for researchers studying oxidative stress-related conditions and seeking to better understand the role of SOD1 in health and disease.
Product Name:
Human SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
SKU:
HUES02204
Target:
Human SOD1 (Superoxide Dismutase 1, Soluble)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
86-101
87-100
Average (%)
105
93
94
1:4
Range (%)
86-98
89-103
95-110
Average (%)
93
94
101
1:8
Range (%)
88-101
88-104
93-106
Average (%)
95
95
99
1:16
Range (%)
85-100
92-107
94-111
Average (%)
92
99
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.3
367.4
1835.4
196.2
331.3
1975.0
Standard deviation
13.2
14.7
55.1
12.8
16.6
75.1
C V (%)
6.31
4.0
3.0
6.52
5.01
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
