Human Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit
The Human Sodium Glucose Cotransporter 2 (SGLT2) ELISA Kit is specifically designed for the accurate measurement of SGLT2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reproducible results for a variety of research applications.SGLT2 is a key protein involved in the regulation of glucose reabsorption in the kidneys, making it a crucial target for diabetes research and drug development.
Monitoring SGLT2 levels can provide valuable insights into glucose metabolism and diabetes-related complications, making this ELISA kit an essential tool for studying diabetes and related conditions.Overall, the Human SGLT2 ELISA Kit offers a reliable and efficient method for quantifying SGLT2 levels in human samples, making it an invaluable resource for researchers and clinicians working in the field of diabetes and metabolic disorders.
Product Name:
Human Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit
Product Code:
HUDL02665
Size:
96 Assays
Target:
SGLT2
Synonyms:
SLC5A2, Solute Carrier Family 5 Member 2, Low affinity sodium-glucose cotransporter, Na(+)/glucose cotransporter 2
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
