Human Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit
The Human Sodium Hydrogen Exchanger 3 (NHE3) ELISA Kit is a highly sensitive and specific assay designed to accurately measure levels of NHE3 in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for various research applications.NHE3 is a vital protein involved in ion transport and pH regulation within cells, playing a crucial role in maintaining cellular homeostasis. Dysregulation of NHE3 has been associated with various diseases such as hypertension, diabetes, and inflammatory bowel disease, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Human NHE3 ELISA Kit provides researchers with a powerful tool to study the role of NHE3 in health and disease, ultimately contributing to a better understanding of cellular physiology and potential treatment strategies.
Product Name:
Human Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit
Product Code:
HUDL02036
Size:
96 Assays
Target:
NHE3
Synonyms:
SLC9A3, Solute Carrier Family 9 Member 3, Sodium-hydrogen antiporter 3, Na(+)/H(+) exchanger 3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
