The Human Sodium Iodide Symporter (NIS) ELISA Kit is a powerful tool for measuring levels of the NIS protein in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The NIS protein is a key player in the active transport of iodide in thyroid follicular cells, making it vital for thyroid hormone synthesis. Dysfunction of the NIS protein can lead to thyroid disorders such as hypothyroidism and goiter.
Understanding NIS expression levels can provide valuable insights into thyroid function and potential treatment options for thyroid-related conditions.Overall, the Human Sodium Iodide Symporter (NIS) ELISA Kit is an indispensable tool for researchers studying thyroid disorders, iodide transport, and thyroid hormone metabolism. Its precision and reliability make it an essential component in advancing our understanding of thyroid biology and developing novel therapies for thyroid diseases.
Product Name:
Human Sodium Iodide Symporter (NIS) ELISA Kit
Product Code:
HUDL02041
Size:
96 Assays
Target:
NIS
Synonyms:
SLC5A5, Solute Carrier Family 5 Member 5, Na(+)/I(-) cotransporter
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
