Human Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit
The Human Solute Carrier Family 30 Member 3 (SLC30A3) ELISA Kit is specifically designed for the detection of SLC30A3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and reliable results for a variety of research purposes.SLC30A3 is a crucial member of the solute carrier family, involved in the transport of zinc ions across cell membranes. Zinc is an essential micronutrient that plays a vital role in various cellular processes, including DNA synthesis, immune function, and wound healing.
Dysregulation of zinc transporters like SLC30A3 has been implicated in diseases such as diabetes, Alzheimer's, and cancer, making it a valuable biomarker for understanding the roles of zinc in these conditions and developing targeted treatments.Overall, the Human SLC30A3 ELISA Kit is a valuable tool for researchers studying zinc transport and its implications in health and disease. Its high performance and accuracy make it an ideal choice for a wide range of applications in the field of molecular biology and biomedical research.
Product Name:
Human Solute Carrier Family 30, Member 3 (SLC30A3) ELISA Kit
Product Code:
HUDL02703
Size:
96 Assays
Target:
SLC30A3
Synonyms:
ZNT3, Zinc Transporter 3
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
