The Human SOST (Sclerostin) CLIA Kit is a reliable and accurate tool for detecting Sclerostin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Sclerostin is a critical regulator of bone metabolism, inhibiting bone formation by blocking the Wnt signaling pathway.
Abnormal levels of Sclerostin have been associated with conditions such as osteoporosis, making it a valuable biomarker for studying bone health and developing potential therapeutic interventions.This CLIA kit provides researchers with a simple and efficient method for measuring Sclerostin levels in human samples, offering valuable insights into bone biology and potential treatment options for bone-related disorders.
Product Name:
Human SOST (Sclerostin) CLIA Kit
SKU:
HUES00874
Target:
Human SOST (Sclerostin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SOST. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOST, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SOST. You can calculate the concentration of Human SOST in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
97-111
100-113
Average (%)
103
103
106
1:4
Range (%)
102-120
93-107
99-109
Average (%)
109
100
104
1:8
Range (%)
99-113
86-101
101-117
Average (%)
105
93
107
1:16
Range (%)
96-108
100-115
95-108
Average (%)
103
107
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
98-111
104
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.91
113.38
374.38
45.99
110.84
390.29
Standard deviation
5.77
12.18
34.85
4.26
10.7
40.04
C V (%)
12.3
10.74
9.31
9.26
9.65
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SOST concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed.
