Human SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit
The Human SPC (Pulmonary Surfactant-Associated Protein C) ELISA Kit is specially designed for the precise detection of SPC levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing dependable and consistent results, making it perfect for a wide array of research endeavors.SPC is a vital protein involved in maintaining the stability of surfactant in the lungs, playing a crucial role in respiratory function.
Imbalances in SPC levels have been linked to various respiratory diseases such as acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, making it a key biomarker for understanding and potentially treating these conditions.In summary, the Human SPC ELISA Kit offers a reliable tool for researchers looking to study and understand the role of SPC in respiratory health and disease.
Product Name:
Human SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit
SKU:
HUES02326
Target:
Human SPC (Pulmonary Surfactant Associated Protein C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SPC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SPC. You can calculate the concentration of Human SPC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
88-99
96-109
Average (%)
102
94
102
1:4
Range (%)
91-102
86-97
84-94
Average (%)
97
91
89
1:8
Range (%)
87-103
80-93
84-94
Average (%)
94
87
89
1:16
Range (%)
91-105
81-90
87-102
Average (%)
99
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-105
100
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
2.67
6.95
1.0
2.86
6.72
Standard deviation
0.07
0.15
0.3
0.07
0.13
0.36
C V (%)
6.86
5.62
4.32
7.0
4.55
5.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SPC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPC in samples. No significant cross-reactivity or interference between Human SPC and analogues was observed.
