Human SPJ (Surfactant Associated Protein J) CLIA Kit
The Human SP-J (Surfactant Associated Protein J) CLIA Kit is a cutting-edge assay designed for the precise measurement of SP-J levels in human samples such as serum and plasma. This advanced kit offers exceptional sensitivity and specificity, delivering accurate and consistent results for a variety of research purposes.SP-J is a vital protein known for its involvement in the regulation of surfactant production in the lungs, essential for maintaining proper respiratory function. Dysregulation of SP-J has been linked to various respiratory conditions such as pulmonary fibrosis and acute respiratory distress syndrome, highlighting its importance as a biomarker for studying these diseases and exploring potential therapeutic interventions.
With its high performance and versatility, the Human SP-J CLIA Kit is a valuable tool for researchers seeking to deepen their understanding of respiratory health and explore new avenues for treatment development. Unlock the potential of SP-J research with this innovative assay kit from Assay Genie.
Product Name:
Human SPJ (Surfactant Associated Protein J) CLIA Kit
SKU:
HUES00635
Target:
Human SPJ (Surfactant Associated Protein J)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPJ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPJ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPJ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPJ. You can calculate the concentration of Human SPJ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
94-107
101-118
Average (%)
109
101
108
1:4
Range (%)
90-104
96-106
90-105
Average (%)
95
101
97
1:8
Range (%)
100-117
93-105
98-114
Average (%)
108
98
105
1:16
Range (%)
92-105
91-104
91-101
Average (%)
99
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
104
EDTA plasma (n=5)
97-110
102
Cell culture media (n=5)
93-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
208.71
577.21
1358.17
196.7
528.9
1459.81
Standard deviation
26.97
49.47
133.78
18.71
53.0
146.42
C V (%)
12.92
8.57
9.85
9.51
10.02
10.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SPJ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPJ in samples. No significant cross-reactivity or interference between Human SPJ and analogues was observed.
