The Human SPTAN1 (Alpha-Fodrin) CLIA Kit from Assaygenie is a cutting-edge tool for the accurate measurement of SPTAN1 levels in human samples. This kit offers superior sensitivity and specificity, guaranteeing precise and consistent results for a variety of research purposes.SPTAN1, also known as Alpha-Fodrin, is a vital protein involved in cell structure and function, playing a critical role in conditions such as neurodegenerative disorders and autoimmune diseases.
Monitoring SPTAN1 levels can provide valuable insights into these conditions and help in the development of targeted therapies.With the Human SPTAN1 (Alpha-Fodrin) CLIA Kit, researchers can confidently analyze SPTAN1 levels in serum, plasma, and cell culture supernatants, paving the way for advancements in the understanding and treatment of various diseases. Choose Assaygenie for reliable and accurate results in your research endeavors.
Product Name:
Human SPTAN1 (Alpha-Fodrin) CLIA Kit
SKU:
HUES00873
Target:
Human SPTAN1 (Alpha-Fodrin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPTAN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPTAN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPTAN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPTAN1. You can calculate the concentration of Human SPTAN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
90-103
87-102
Average (%)
92
96
93
1:4
Range (%)
90-102
87-104
101-117
Average (%)
95
95
109
1:8
Range (%)
92-106
96-107
102-114
Average (%)
99
102
108
1:16
Range (%)
88-105
97-113
102-116
Average (%)
96
103
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.49
197.89
674.08
92.88
196.13
734.46
Standard deviation
11.86
15.91
54.33
10.39
21.48
76.97
C V (%)
12.29
8.04
8.06
11.19
10.95
10.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SPTAN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPTAN1 in samples. No significant cross-reactivity or interference between Human SPTAN1 and analogues was observed.
