Human SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit
The Human SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit is specially designed for the precise quantification of SREBP levels in human serum, plasma, and tissue culture supernatants. With its superior sensitivity and specificity, this kit delivers consistent and trustworthy results, making it perfect for a diverse range of research applications.SREBPs are pivotal transcription factors that regulate lipid metabolism and cellular homeostasis.
Dysregulation of SREBP activity is associated with numerous diseases such as obesity, diabetes, and cardiovascular disorders, highlighting the significance of SREBPs as biomarkers in disease research and therapeutic development.Order the Human SREBP ELISA Kit now from Assay Genie to advance your research in lipid metabolism and related diseases.
Product Name:
Human SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit
SKU:
HUES02242
Target:
Human SREBP (Sterol Regulatory Element Binding Proteins)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SREBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SREBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SREBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SREBP. You can calculate the concentration of Human SREBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
86-99
85-96
Average (%)
93
93
90
1:4
Range (%)
92-106
82-95
85-97
Average (%)
98
88
90
1:8
Range (%)
85-99
85-97
88-101
Average (%)
92
91
94
1:16
Range (%)
88-100
82-94
86-100
Average (%)
94
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
87-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.38
8.55
1.04
2.52
8.38
Standard deviation
0.05
0.13
0.47
0.05
0.13
0.32
C V (%)
5.26
5.46
5.5
4.81
5.16
3.82
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SREBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SREBP in samples. No significant cross-reactivity or interference between Human SREBP and analogues was observed.
