The Human SST (Somatostatin) ELISA Kit is a reliable and accurate tool for detecting levels of somatostatin in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for a variety of research applications.Somatostatin is a key hormone that regulates various physiological functions, including inhibiting the release of growth hormone, insulin, and glucagon.
It also plays a role in modulating neurotransmission and cell proliferation. Dysregulation of somatostatin levels has been associated with several disorders, such as growth hormone disorders, diabetes, and certain tumors.This ELISA kit offers researchers a valuable tool for studying the role of somatostatin in health and disease, allowing for the development of potential diagnostic and therapeutic strategies. Discover more about the Human SST ELISA Kit at www.assaygenie.com.
Product Name:
Human SST (Somatostatin) ELISA Kit
SKU:
HUES02869
Target:
Human SST (Somatostatin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SST. During the reaction, SST in the sample or standard competes with a fixed amount of SST on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SST. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SST in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
100-116
97-110
Average (%)
104
106
103
1:4
Range (%)
88-100
91-102
96-110
Average (%)
95
97
104
1:8
Range (%)
86-96
91-106
98-115
Average (%)
91
97
106
1:16
Range (%)
84-99
90-105
102-113
Average (%)
90
97
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
96
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.2
66.9
217.4
21.4
63.2
228.6
Standard deviation
1.6
4.0
10.0
1.2
2.7
11.2
C V (%)
6.9
5.98
4.6
5.61
4.27
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes SST in samples. No significant cross-reactivity or interference between SST and analogues was observed.
