Human Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit
The Human Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit is specifically designed for the precise measurement of STAR levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistently accurate and dependable results, making it an indispensable tool for various research purposes.STAR, also known as StAR, is a key protein involved in steroidogenesis, which is the process of steroid hormone production in the body. This protein is essential for the transport of cholesterol into mitochondria, where it is converted into steroid hormones, such as cortisol, aldosterone, and sex hormones.
Dysregulation of STAR expression can lead to various endocrine disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human STAR ELISA Kit offers researchers a reliable and efficient method for analyzing STAR levels in biological samples, providing valuable insights into steroid hormone production and related disorders.
Product Name:
Human Steroidogenic Acute Regulatory Protein (STAR) ELISA Kit
Product Code:
HUDL02782
Size:
96 Assays
Target:
STAR
Synonyms:
STARD1, START domain-containing protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
