Human STUB1 (E3 ubiquitin-protein ligase CHIP) ELISA Kit
The Human STUB1 (STIP1 Homology and U-Box Containing Protein 1) E3 Ubiquitin Protein Ligase Chip ELISA Kit is specially designed for the accurate detection of STUB1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.STUB1 is an important protein with E3 ubiquitin ligase activity, playing a key role in regulating protein degradation and cellular homeostasis. Dysregulation of STUB1 has been linked to various diseases, including neurodegenerative disorders, cancer, and inflammatory conditions.
As such, measuring STUB1 levels can provide valuable insights into disease pathology and potential therapeutic targets.With its user-friendly protocol and high-quality components, the Human STUB1 E3 Ubiquitin Protein Ligase Chip ELISA Kit from Assay Genie is a valuable tool for researchers and healthcare professionals looking to study the role of STUB1 in disease development and progression. Order your kit today and unlock the potential of STUB1 research.
Product Name:
Human STUB1 (E3 ubiquitin-protein ligase CHIP) ELISA Kit
SKU:
HUES03512
Target:
Human STUB1 (E3 ubiquitin-protein ligase CHIP) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human STUB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human STUB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human STUB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human STUB1. You can calculate the concentration of Human STUB1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
89-105
98-115
Average (%)
100
96
105
1:4
Range (%)
93-106
87-98
84-95
Average (%)
98
92
90
1:8
Range (%)
87-101
81-95
86-99
Average (%)
94
87
93
1:16
Range (%)
86-100
85-96
83-96
Average (%)
93
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
98
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.62
307.91
875.56
102.66
293.71
958.48
Standard deviation
6.34
12.69
33.36
5.4
15.1
52.43
C V (%)
6.7
4.12
3.81
5.26
5.14
5.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human STUB1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human STUB1 in samples. No significant cross-reactivity or interference between Human STUB1 and analogues was observed.
