Human Sulfatase Modifying Factor 1 (SUMF1) ELISA Kit
The Human Sulfatase Modifying Factor 1 (SUMF1) ELISA Kit is specifically designed for the precise measurement of SUMF1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results, making it well-suited for various research purposes.SUMF1 is a critical enzyme involved in sulfate metabolism and modification, playing a key role in the synthesis of sulfatase enzymes.
Dysregulation of SUMF1 has been associated with various diseases including lysosomal storage disorders and sulfatase deficiency syndromes. This makes it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human SUMF1 ELISA Kit provides researchers with a reliable tool to investigate the role of SUMF1 in health and disease, offering valuable insights for the development of novel diagnostic and therapeutic strategies.
Product Name:
Human Sulfatase Modifying Factor 1 (SUMF1) ELISA Kit
Product Code:
HUDL02805
Size:
96 Assays
Target:
SUMF1
Synonyms:
FGE, C-alpha-formylglycine-generating enzyme 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
