Human SUMO2 (Small Ubiquitin Related Modifier 2) CLIA Kit
The Human SUMO2 (Small Ubiquitin-related Modifier 2) CLIA Kit is specifically designed for the accurate quantification of SUMO2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SUMO2 is a key player in the ubiquitin-like protein modification pathway, regulating various cellular processes including protein stability, localization, and interactions. Dysregulation of SUMOylation has been implicated in various diseases such as cancer, neurodegenerative disorders, and autoimmune diseases, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.
The Human SUMO2 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying SUMO2 biology and its role in human health and disease. With its user-friendly protocol and accurate results, this kit is a valuable addition to any laboratory conducting research in the field of protein modification and disease mechanisms.
Product Name:
Human SUMO2 (Small Ubiquitin Related Modifier 2) CLIA Kit
SKU:
HUES01141
Target:
Human SUMO2 (Small Ubiquitin Related Modifier 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SUMO2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SUMO2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SUMO2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SUMO2. You can calculate the concentration of Human SUMO2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-121
93-107
101-118
Average (%)
110
99
108
1:4
Range (%)
103-117
96-113
92-106
Average (%)
109
104
99
1:8
Range (%)
93-106
100-112
93-106
Average (%)
100
105
100
1:16
Range (%)
92-106
87-98
100-115
Average (%)
99
92
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.57
464.23
1696.2
197.99
465.85
1849.81
Standard deviation
15.52
44.71
188.45
23.22
40.67
124.68
C V (%)
8.06
9.63
11.11
11.73
8.73
6.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SUMO2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SUMO2 in samples. No significant cross-reactivity or interference between Human SUMO2 and analogues was observed.
