The Human Survivin CLIA Kit is specifically designed for the precise measurement of survivin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Survivin is a key protein known for its role in regulating cell division and apoptosis. Its overexpression has been linked to various cancers, including breast, lung, and colon cancer, making it a valuable biomarker for cancer research and potential therapeutic developments.
Understanding survivin levels can provide crucial insights into cancer progression and treatment strategies.With its advanced technology and proven performance, the Human Survivin CLIA Kit is an essential tool for researchers and clinicians investigating survivin's involvement in cancer biology and other disease processes. Trust in this kit to deliver reliable data for your scientific inquiries and medical advancements.
Product Name:
Human Surv (Survivin) CLIA Kit
SKU:
HUES00900
Target:
Human Surv (Survivin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Surv. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Surv and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Surv, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Surv. You can calculate the concentration of Human Surv in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
100-115
90-101
Average (%)
104
109
95
1:4
Range (%)
92-103
87-98
93-104
Average (%)
97
93
98
1:8
Range (%)
86-101
98-110
86-100
Average (%)
92
105
93
1:16
Range (%)
97-109
96-109
92-104
Average (%)
103
102
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
100-118
108
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.01
121.63
375.08
47.15
120.36
401.21
Standard deviation
4.53
11.64
34.96
5.44
12.11
32.26
C V (%)
9.24
9.57
9.32
11.54
10.06
8.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Surv concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Surv in samples. No significant cross-reactivity or interference between Human Surv and analogues was observed.
