The Human Syncollin CLIA Kit is a cutting-edge assay designed for the precise measurement of syncollin levels in human biological samples such as serum, plasma, and cell culture supernatants. This state-of-the-art kit offers unparalleled sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Syncollin is a key protein involved in insulin secretion and plays a crucial role in regulating glucose metabolism. Dysregulation of syncollin has been implicated in various metabolic disorders, making it a valuable biomarker for studying conditions such as diabetes and obesity.
By measuring syncollin levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially discover new therapeutic targets.Overall, the Human Syncollin CLIA Kit is an indispensable tool for researchers looking to investigate the role of syncollin in metabolic regulation and develop innovative treatments for related disorders. With its exceptional performance and ease of use, this kit is a must-have for any laboratory conducting cutting-edge research in the field of metabolic health.
Product Name:
Human SYCN (Syncollin) CLIA Kit
SKU:
HUES00593
Target:
Human SYCN (Syncollin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SYCN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SYCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SYCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SYCN. You can calculate the concentration of Human SYCN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
100-114
85-98
Average (%)
101
106
91
1:4
Range (%)
94-111
95-109
98-113
Average (%)
102
102
105
1:8
Range (%)
90-104
101-117
85-99
Average (%)
95
108
92
1:16
Range (%)
90-104
89-101
96-109
Average (%)
95
96
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
100
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.54
273.36
794.56
111.1
246.35
808.74
Standard deviation
12.41
31.05
86.29
12.8
19.71
55.8
C V (%)
12.1
11.36
10.86
11.52
8.0
6.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SYCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SYCN in samples. No significant cross-reactivity or interference between Human SYCN and analogues was observed.
