Human TBP2 (Thioredoxin Binding Protein 2) CLIA Kit
The Human TBP2 (Thioredoxin Binding Protein 2) CLIA Kit is specifically designed for the accurate quantification of TBP2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.TBP2, also known as thioredoxin binding protein 2, plays a crucial role in regulating cellular redox balance and modulating cell proliferation.
Dysregulation of TBP2 has been linked to various diseases such as cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With the Human TBP2 CLIA Kit, researchers can confidently measure TBP2 levels in biological samples with ease and accuracy, providing valuable insights into the role of TBP2 in disease pathogenesis and progression.
Product Name:
Human TBP2 (Thioredoxin Binding Protein 2) CLIA Kit
SKU:
HUES00967
Target:
Human TBP2 (Thioredoxin Binding Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TBP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TBP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TBP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TBP2. You can calculate the concentration of Human TBP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
86-99
87-99
Average (%)
107
93
92
1:4
Range (%)
98-111
94-109
102-118
Average (%)
105
101
109
1:8
Range (%)
95-110
90-103
86-98
Average (%)
103
97
93
1:16
Range (%)
97-113
94-111
93-110
Average (%)
104
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
94-104
99
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.35
305.25
669.27
94.14
280.7
672.07
Standard deviation
8.99
25.82
65.92
11.0
25.82
57.66
C V (%)
9.43
8.46
9.85
11.68
9.2
8.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TBP2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed.
