Human TCC C5b-9 (Terminal Complement Complex C5b-9) CLIA Kit
The Human TCC (C5b-9) Terminal Complement Complex CLIA Kit is specifically designed for the accurate measurement of TCC (C5b-9) levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.The terminal complement complex (TCC) is a key component of the complement system, playing a crucial role in immune defense and inflammation. Abnormal levels of TCC have been associated with various autoimmune disorders, inflammatory conditions, and infectious diseases, making it an important biomarker for studying these diseases and developing potential therapeutic interventions.
With its advanced technology and ease of use, the Human TCC (C5b-9) Terminal Complement Complex CLIA Kit is an invaluable tool for researchers and clinicians working in the fields of immunology, inflammation, and infectious diseases. Order now and unlock new insights into the role of TCC in human health and disease.
Product Name:
Human TCC C5b-9 (Terminal Complement Complex C5b-9) CLIA Kit
SKU:
HUES00978
Target:
Human TCC C5b-9 (Terminal Complement Complex C5b-9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human sC5b-9. You can calculate the concentration of Human sC5b-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
91-105
93-107
Average (%)
106
99
100
1:4
Range (%)
87-100
87-101
100-111
Average (%)
94
94
105
1:8
Range (%)
97-110
101-116
91-103
Average (%)
103
106
98
1:16
Range (%)
93-108
100-116
102-117
Average (%)
101
106
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
101-116
107
Cell culture media (n=5)
84-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.54
157.53
486.22
54.06
159.98
462.72
Standard deviation
5.21
11.99
52.41
5.65
13.13
53.12
C V (%)
9.73
7.61
10.78
10.45
8.21
11.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human sC5b-9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sC5b-9 in samples. No significant cross-reactivity or interference between Human sC5b-9 and analogues was observed.
