Human TDP43 (Tar DNA Binding Protein 43KDa) CLIA Kit
The Human TDP43 (TAR DNA-binding protein 43kDa) CLIA Kit is a highly accurate and sensitive assay designed for the detection of TDP43 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and reproducibility, ensuring reliable results for a variety of research applications.TDP43 is a key protein involved in RNA processing and regulation, with implications in neurological disorders such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD).
By accurately measuring TDP43 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human TDP43 CLIA Kit from Assay Genie provides researchers with a powerful tool for studying the role of TDP43 in disease pathology and developing new strategies for diagnosis and treatment.
Product Name:
Human TDP43 (Tar DNA Binding Protein 43KDa) CLIA Kit
SKU:
HUES00561
Target:
Human TDP43 (Tar DNA Binding Protein 43KDa )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TDP43. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TDP43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TDP43, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TDP43. You can calculate the concentration of Human TDP43 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
94-107
99-114
Average (%)
110
99
107
1:4
Range (%)
96-111
90-104
100-117
Average (%)
102
95
107
1:8
Range (%)
91-106
96-109
86-100
Average (%)
99
102
93
1:16
Range (%)
98-113
102-115
92-104
Average (%)
104
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
98-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.36
635.1
1973.5
219.64
588.14
2132.62
Standard deviation
19.35
50.74
207.61
25.9
54.99
172.96
C V (%)
8.78
7.99
10.52
11.79
9.35
8.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TDP43 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TDP43 in samples. No significant cross-reactivity or interference between Human TDP43 and analogues was observed.
