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Human Tensin 3 (TNS3) ELISA Kit (HUDL02956)

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SKU:
HUDL02956
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q68CZ2
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TENS1,TEM6,TPP,Tumor Endothelial Marker 6,Tensin-Like SH2 Domain-Containing 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649

Description

system_update_altDatasheet

Human Tensin 3 (TNS3) ELISA Kit

The Human TNS3 (Tensin-3) ELISA Kit is a reliable and precise tool for detecting Tensin-3 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.Tensin-3 is an important protein involved in cell adhesion and migration processes, playing a key role in cellular signaling pathways.

Dysregulation of Tensin-3 has been linked to various diseases such as cancer, cardiovascular disorders, and neurological conditions, making it a valuable biomarker for exploring disease mechanisms and potential therapeutic interventions.Overall, the Human TNS3 ELISA Kit is a valuable tool for researchers studying Tensin-3 biology and its implications in human health and disease.

Product Name:Human Tensin 3 (TNS3) ELISA Kit
Product Code:HUDL02956
Size:96 Assays
Target:TNS3
Synonyms:TENS1, TEM6, TPP, Tumor Endothelial Marker 6, Tensin-Like SH2 Domain-Containing 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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