The Human TF (Transferrin) CLIA Kit is a cutting-edge assay designed for the accurate measurement of transferrin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.Transferrin is a key protein responsible for iron transport in the body, playing a vital role in maintaining iron homeostasis. Imbalances in transferrin levels have been associated with a range of health conditions, including anemia, hemochromatosis, and liver diseases.
As such, this kit serves as a valuable tool for investigating these conditions and developing potential therapeutic interventions.With its user-friendly protocol and reliable performance, the Human TF CLIA Kit is an indispensable resource for researchers and clinicians seeking to understand the role of transferrin in health and disease. Order yours today to unlock new insights into iron metabolism and related disorders.
Product Name:
Human TF (Transferrin) CLIA Kit
SKU:
HUES00147
Target:
Human TF (Transferrin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TRF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TRF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TRF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TRF. You can calculate the concentration of Human TRF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
89-103
101-115
Average (%)
90
96
109
1:4
Range (%)
89-101
100-118
88-100
Average (%)
96
109
95
1:8
Range (%)
95-108
91-104
88-99
Average (%)
102
98
94
1:16
Range (%)
99-112
93-107
95-112
Average (%)
105
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
102-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.96
2.7
7.33
1.04
2.69
7.41
Standard deviation
0.09
0.26
0.54
0.13
0.24
0.56
C V (%)
9.38
9.63
7.37
12.5
8.92
7.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TRF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TRF in samples. No significant cross-reactivity or interference between Human TRF and analogues was observed.
