The Human TFF1 (Trefoil Factor 1) CLIA Kit is specifically designed for the accurate measurement of TFF1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.TFF1, also known as trefoil factor 1, is an important protein that plays a key role in mucosal protection and repair. It is involved in maintaining the integrity of the gastrointestinal mucosa and has been linked to conditions such as inflammatory bowel disease and gastric cancer.
By measuring TFF1 levels, researchers can gain insights into the pathophysiology of these diseases and potentially identify new therapeutic targets.Overall, the Human TFF1 CLIA Kit is a valuable tool for researchers studying mucosal integrity, gastrointestinal diseases, and related conditions, providing accurate measurements of TFF1 levels to drive forward scientific discoveries and potential clinical applications.
Product Name:
Human TFF1 (Trefoil Factor 1) CLIA Kit
SKU:
HUES01035
Target:
Human TFF1 (Trefoil Factor 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFF1. You can calculate the concentration of Human TFF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
100-116
88-101
Average (%)
99
106
96
1:4
Range (%)
96-111
83-96
84-99
Average (%)
103
90
91
1:8
Range (%)
97-113
97-110
92-106
Average (%)
105
104
99
1:16
Range (%)
97-113
91-108
100-117
Average (%)
103
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
95-110
100
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.51
279.83
966.11
110.09
291.33
971.25
Standard deviation
11.57
19.84
81.54
13.89
21.35
97.13
C V (%)
11.07
7.09
8.44
12.62
7.33
10.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFF1 in samples. No significant cross-reactivity or interference between Human TFF1 and analogues was observed.
