The Human TFR2 (Transferrin Receptor 2) CLIA Kit is specifically designed for the precise measurement of TFR2 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.TFR2 is a critical protein involved in iron homeostasis and regulation, playing a key role in cellular iron uptake and transferrin binding. Dysregulation of TFR2 has been implicated in various diseases, including hereditary hemochromatosis and liver disorders, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic strategies.
With its reliable performance and ease of use, the Human TFR2 CLIA Kit is an essential tool for researchers looking to investigate the role of TFR2 in health and disease, paving the way for new insights and advancements in the field of iron metabolism and related disorders.
Product Name:
Human TFR2 (Transferrin Receptor 2) CLIA Kit
SKU:
HUES01214
Target:
Human TFR2 (Transferrin Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TFR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TFR2. You can calculate the concentration of Human TFR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
88-100
93-106
Average (%)
109
93
98
1:4
Range (%)
98-114
90-106
97-112
Average (%)
104
96
105
1:8
Range (%)
93-106
97-112
84-97
Average (%)
100
103
91
1:16
Range (%)
99-112
87-99
96-108
Average (%)
107
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
106
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
97-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.01
286.16
867.9
109.04
295.7
854.93
Standard deviation
10.28
27.61
95.47
12.23
22.24
79.17
C V (%)
9.52
9.65
11.0
11.22
7.52
9.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TFR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFR2 in samples. No significant cross-reactivity or interference between Human TFR2 and analogues was observed.
