The Human TG (Thyroglobulin) CLIA Kit is specifically developed for the precise measurement of thyroglobulin levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees dependable and consistent results, making it a valuable tool for various research purposes.Thyroglobulin is a key protein involved in thyroid function, serving as a precursor for thyroid hormone synthesis. Abnormal thyroglobulin levels can indicate thyroid disorders such as cancer or autoimmune diseases, making it a crucial biomarker for studying these conditions and monitoring treatment effectiveness.
With the Human TG (Thyroglobulin) CLIA Kit, researchers can accurately measure thyroglobulin levels and gain valuable insights into thyroid health and related conditions. Upgrade your research capabilities with this advanced kit and unlock new possibilities in the field of endocrinology.
Product Name:
Human TG (Thyroglobulin) CLIA Kit
SKU:
HUES00846
Target:
Human TG (Thyroglobulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
89-101
98-115
Average (%)
110
94
105
1:4
Range (%)
99-113
93-105
96-111
Average (%)
106
100
101
1:8
Range (%)
88-101
89-100
89-103
Average (%)
95
95
96
1:16
Range (%)
99-114
98-110
85-98
Average (%)
105
104
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
89-106
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.18
176.4
862.69
99.11
185.46
851.6
Standard deviation
12.48
17.15
93.17
11.56
17.82
61.06
C V (%)
12.1
9.72
10.8
11.66
9.61
7.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TG in samples. No significant cross-reactivity or interference between Human TG and analogues was observed.
