Human TGF- beta2 (Transforming Growth Factor beta2) CLIA Kit
The Human TGF-Beta2 (Transforming Growth Factor Beta2) CLIA Kit is specifically designed for the accurate detection of TGF-Beta2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TGF-Beta2 is a key protein involved in the regulation of cell growth, differentiation, and the immune response. It plays a critical role in various biological processes, including wound healing, embryonic development, and the pathogenesis of diseases such as cancer and fibrosis.
As a crucial biomarker, studying TGF-Beta2 levels can provide valuable insights into these conditions and potential therapeutic interventions.Overall, the Human TGF-Beta2 CLIA Kit from Assay Genie is an essential tool for researchers looking to accurately measure TGF-Beta2 levels and further understand its role in health and disease. With its high performance and user-friendly design, this kit is suitable for a wide range of experimental settings.
Product Name:
Human TGF- beta2 (Transforming Growth Factor beta2) CLIA Kit
SKU:
HUES00903
Target:
Human TGF- beta2 (Transforming Growth Factor beta2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TGF-β2. You can calculate the concentration of Human TGF-β2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
88-102
84-100
Average (%)
95
95
91
1:4
Range (%)
95-108
94-109
92-107
Average (%)
102
102
97
1:8
Range (%)
100-113
86-100
89-102
Average (%)
106
92
95
1:16
Range (%)
92-104
89-101
84-98
Average (%)
97
95
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.53
83.87
394.01
38.54
92.24
398.33
Standard deviation
4.26
9.97
30.02
3.78
7.29
41.51
C V (%)
10.02
11.89
7.62
9.81
7.9
10.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TGF-β2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-β2 in samples. No significant cross-reactivity or interference between Human TGF-β2 and analogues was observed.
