Human TGF- beta2 (Transforming Growth Factor beta2) ELISA Kit
The Human TGF-Beta2 (Transforming Growth Factor-Beta2) ELISA Kit is specifically designed for the accurate and precise measurement of TGF-Beta2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.TGF-Beta2 is a key growth factor that regulates cell growth, proliferation, differentiation, and apoptosis. It plays a critical role in various cellular processes, including immune response, tissue repair, and development.
Dysregulation of TGF-Beta2 has been linked to a range of diseases, such as cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.With its reliable performance and user-friendly format, the Human TGF-Beta2 ELISA Kit from Assay Genie is an essential tool for researchers seeking to explore the role of TGF-Beta2 in health and disease.
Product Name:
Human TGF- beta2 (Transforming Growth Factor beta2) ELISA Kit
SKU:
HUES02597
Target:
Human TGF- beta2 (Transforming Growth Factor beta2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGF-β2. You can calculate the concentration of Human TGF-β2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
87-100
90-104
Average (%)
90
94
95
1:4
Range (%)
92-108
81-91
87-99
Average (%)
98
86
94
1:8
Range (%)
92-106
86-100
83-95
Average (%)
100
91
88
1:16
Range (%)
88-101
83-97
83-94
Average (%)
95
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
93-110
100
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.16
263.47
859.83
114.83
248.33
855.61
Standard deviation
6.81
11.33
32.33
5.76
12.44
26.35
C V (%)
6.18
4.3
3.76
5.02
5.01
3.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TGF-β2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-β2 in samples. No significant cross-reactivity or interference between Human TGF-β2 and analogues was observed.
