The Human TGF-beta1 (Transforming Growth Factor Beta-Induced Protein) CLIA Kit is designed for the precise quantification of TGF-beta1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TGF-beta1 is a key regulator of cell growth, differentiation, and immune response, playing a critical role in various physiological and pathological processes.
Dysregulation of TGF-beta1 signaling has been implicated in diseases such as cancer, fibrosis, and autoimmune disorders, making it a valuable target for research and therapeutic development.With the Human TGF-beta1 CLIA Kit, researchers can confidently measure TGF-beta1 levels in biological samples, providing valuable insights into disease mechanisms and potential treatment strategies. Trust AssayGenie for reliable and high-quality tools for your research needs.
Human TGF betaI (Transforming Growth Factor Beta Induced Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TGFβI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGFβI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGFβI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TGFβI. You can calculate the concentration of Human TGFβI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
89-102
93-106
Average (%)
94
97
100
1:4
Range (%)
96-107
91-104
85-95
Average (%)
102
96
90
1:8
Range (%)
98-113
101-117
102-120
Average (%)
105
109
110
1:16
Range (%)
104-120
92-104
101-116
Average (%)
110
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
98-112
106
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
425.31
829.69
3176.19
448.82
895.81
3347.96
Standard deviation
41.08
91.76
365.26
51.17
65.04
264.49
C V (%)
9.66
11.06
11.5
11.4
7.26
7.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TGFβI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGFβI in samples. No significant cross-reactivity or interference between Human TGFβI and analogues was observed.
