The Human TGM2 (Transglutaminase 2) Tissue CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of TGM2 levels in human tissue samples. This kit provides researchers with reliable and reproducible results, making it ideal for a variety of research applications.Transglutaminase 2 is an important enzyme involved in various cellular processes, including cell differentiation, apoptosis, and cell migration. Dysregulation of TGM2 has been implicated in a number of diseases, including cancer, celiac disease, and neurodegenerative disorders, highlighting its significance as a potential biomarker for disease diagnosis and treatment.
With its high sensitivity and specificity, the Human TGM2 Tissue CLIA Kit offers researchers a valuable tool for studying the role of transglutaminase 2 in health and disease, providing insights that may lead to the development of novel therapeutic strategies.
Product Name:
Human TGM2 (Transglutaminase 2, Tissue) CLIA Kit
SKU:
HUES01209
Target:
Human TGM2 (Transglutaminase 2, Tissue)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TGM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TGM2. You can calculate the concentration of Human TGM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
94-111
85-96
Average (%)
108
101
90
1:4
Range (%)
88-103
94-108
91-102
Average (%)
95
100
97
1:8
Range (%)
97-112
99-114
99-113
Average (%)
105
105
105
1:16
Range (%)
88-101
94-109
91-106
Average (%)
95
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.24
199.01
925.97
100.67
188.15
1012.3
Standard deviation
12.06
20.42
61.95
9.8
16.03
74.4
C V (%)
12.93
10.26
6.69
9.73
8.52
7.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TGM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGM2 in samples. No significant cross-reactivity or interference between Human TGM2 and analogues was observed.
