Description
Human TGM3/Transglutaminase 3, Epidermal ELISA Kit
The Human TGM3 (Transglutaminase 3) ELISA Kit is specially designed for the precise measurement of TGM3 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and repeatable results, making it an excellent choice for a variety of research applications.TGM3, also known as transglutaminase 3, is a key enzyme involved in skin barrier function and epidermal differentiation. Dysregulation of TGM3 has been linked to various skin disorders and conditions, highlighting its importance as a potential biomarker for studying skin health and developing therapeutic interventions.
This ELISA kit provides researchers with a reliable tool for investigating the role of TGM3 in skin biology, dermatology, and related fields. Its easy-to-use format and robust performance make it an invaluable resource for advancing our understanding of TGM3 function and its implications for human health.
| Product Name: | Human TGM3/Transglutaminase 3, Epidermal ELISA Kit |
| Product Code: | HUFI02899 |
| Size: | 96 Assays |
| Alias: | TGM3, TG3, TgaseE, EC 2.3.2.13, MGC126250, protein-glutamine gamma-glutamyltransferase E, TG, E, TGase E, TGase-3, TGEMGC126249, transglutaminase 3, E polypeptide, protein-glutamine-gamma-glutamyltransferase, Transglutaminase E, Transglutaminase-3, transglutaminase-3 |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human TGM3 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.188ng/ml |
| Range: | 0.313-20ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human TGM3 and the recovery rates were calculated by comparing the measured value to the expected amount of Human TGM3 in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human TGM3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | Q08188 |
| UniProt Protein Function: | TGM3: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross- linking structural proteins to hardening the inner root sheath. Belongs to the transglutaminase superfamily. Transglutaminase family. |
| UniProt Protein Details: | Protein type:EC 2.3.2.13; Membrane protein, peripheral; Transferase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm Molecular Function:protein-glutamine gamma-glutamyltransferase activity; transferase activity, transferring acyl groups; calcium ion binding; catalytic activity Biological Process: keratinocyte differentiation; cell envelope organization and biogenesis; hair follicle morphogenesis; keratinization; protein modification process; peptide cross-linking; protein tetramerization |
| NCBI Summary: | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q08188 |
| NCBI GenInfo Identifier: | 257051080 |
| NCBI Gene ID: | 7053 |
| NCBI Accession: | Q08188.4 |
| UniProt Secondary Accession: | Q08188,O95933, Q32ML9, Q32MM0, A8K5N6, B2RCR6, D3DVX1 |
| UniProt Related Accession: | Q08188 |
| Molecular Weight: | |
| NCBI Full Name: | Protein-glutamine gamma-glutamyltransferase E |
| NCBI Synonym Full Names: | transglutaminase 3 |
| NCBI Official Symbol: | TGM3 |
| NCBI Official Synonym Symbols: | TGE |
| NCBI Protein Information: | protein-glutamine gamma-glutamyltransferase E; TG(E); TGase E; TGase-3; transglutaminase E; transglutaminase-3; E polypeptide, protein-glutamine-gamma-glutamyltransferase |
| UniProt Protein Name: | Protein-glutamine gamma-glutamyltransferase E |
| UniProt Synonym Protein Names: | Transglutaminase E; TG(E); TGE; TGase E; Transglutaminase-3; TGase-3 |
| Protein Family: | Protein-glutamine gamma-glutamyltransferase |
| UniProt Gene Name: | TGM3 |
| UniProt Entry Name: | TGM3_HUMAN |
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Protocol |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Aliquot 0.1ml standard solutions into the standard wells. |
| 3. | Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well. |
| 4. | Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells. |
| 5. | Seal the plate with a cover and incubate at 37 °C for 90 min. |
| 6. | Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2. |
| 7. | Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall. |
| 8. | Seal the plate with a cover and incubate at 37°C for 60 min. |
| 9. | Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash. |
| 10. | Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min. |
| 11. | Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min. |
| 12. | Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color. |
| 13. | Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately. |
| 14. | Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
